A vitamin is an organic compound that the body requires in lesser amounts to sustain essential functions and maintain overall health. These compounds are vital because the body either cannot produce them at all or cannot produce enough of them, so it is required to be obtained through diet.
Water-soluble vitamins are a group of essential nutrients that dissolve in water and are not stored in the body to any significant extent. Since the body does not stockpile these vitamins, it is important to consume them regularly through diet or supplements. The water-soluble vitamins are – Vitamin C (Ascorbic acid), Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Vitamin B3 (Niacin and Niacinamide), Vitamin B5 (Pantothenic Acid), Vitamin B6 (Pyridoxine), Vitamin B7 (Biotin), Vitamin B9 (Folic Acid), Vitamin B12 (Cyanocobalamin).
For the analysis of the various vitamins on the samples, it is important to select the appropriate method for analysis. Selection of method of analysis is based on the sample type and level of vitamin in the samples. The laboratory has set up via validation and verification procedures – various analytical methods for use by customers.
Vitamin B1, B2, B3 and B6: AsureQuality has verified/ validated the Global Standard method referenced to AOAC 2015.14 an LC MS/MS method for the quantification of Total Vitamin B1, Total Vitamin B2, Total Vitamin B3 and Total Vitamin B6 in various matrices such as milk based infant formula and related nutritional products such as adult nutritional formula, goat and sheep milk powder based products, including cow milk, cream and other liquid based products, wide range of food matrices, including but not limited to honey, vegetables, meat and meat products, fish and fish products, beverages, egg and egg products, bread, dietary supplements and pet foods.
For the analysis of premixes, raw materials and dietary supplements typically containing individual level of vitamins greater than 1 mg/g for Vitamin B2, B6 and greater than 10mg/g for Vitamin B3, the laboratory has validated an LC based method referenced to Journal of Chromatography, Volume 301 (1984) for use.
Vitamin B5: AsureQuality has verified/ validated the Global Standard method referenced to AOAC 2012.16 an LC MS/MS method for the quantification of Pantothenic acid in infant formula and adult/paediatric nutritional formula, dietary supplements, beverages, pet food, feeds, and other foods.
For the analysis of premixes, raw materials and dietary supplements typically containing level of vitamins greater than 0.5 mg/g for Vitamin B5, the laboratory has validated an LC based method referenced to JAOAC Volume 67 – 994-998 (1984) – Bristol Myers Method G7270b for use.
Vitamin B9 (folic acid): AsureQuality has verified/ validated the Global Standard method referenced to AOAC 2013.13 an LC MS/MS method for the quantification of Folic Acid in infant formula, adult/paediatric nutritional formula, including goat and sheep milk powder-based products, raw milk, UHT milk and soy milk, food, pet food, dietary supplements and pharmaceuticals.
For the analysis of premixes, raw materials and dietary supplements typically containing level of vitamins greater than 0 0.05 mg/g for Folic acid, the laboratory has validated an LC based method referenced to JAOAC Volume 67 – 994-998 (1984) – Bristol Myers Method G7270b for use.
Vitamin B7 (Biotin): AsureQuality has verified/ validated the Global Standard method referenced to AOAC 2016.02 and AOAC 2016.02 (modified), an LC / UHPLC method for the quantification of Biotin in infant formula and adult/paediatric nutritional formula, liquid milk and milk powders, caprine/ovine milk, whey protein concentrates, premix and dietary supplements.
For the analysis of premixes, raw materials and dietary supplements typically containing level of vitamins greater than 0.012 mg/g for Biotin, the laboratory has validated an LC based method referenced to JAOAC Volume 67 – 994-998 (1984) – Bristol Myers Method G7270b for use.
Vitamin B12 (Cyanocobalamin): AsureQuality has verified/ validated the Global Standard method referenced to AOAC 2014.02 and AOAC 2014.02 (modified), an UHPLC method for the quantification of Cyanocobalamin in infant formula and adult/paediatric nutritional formula, liquid milk and milk powders, whey protein concentrates, goat milk, cream, beverages, meat, pharmaceutical nutritional powders, premix and dietary supplements.
For the analysis of premixes, raw materials and dietary supplements typically containing level of vitamins greater than 2 µg/g for Vitamin B12, the laboratory has validated an LC based method referenced to
JAOAC Volume 67 for use.
More information
For further detail about vitamin B testing, please read: Vitamin B1, B2, B3, and B6 analysis (customer sign in required)
Testing is conducted in the Chemistry department at our Auckland Laboratory.
Samples should be transported in a light-proof container or bags. Liquid milk and other perishable items must be chilled. If the samples are to be analysed for all the B group vitamins, 250 grams of sample is required for analysis.
Please pre-register your samples in LabConnect or read more about manual sample submission.