New UPLC method for simultaneous determination of caffeine and theobromine

We’ve introduced a new ultra-high-performance liquid chromatography (UPLC) method for the simultaneous determination of caffeine and theobromine.

This method provides:

• Simultaneous determination of both compounds – caffeine and theobromine analysed together.
• Application across diverse matrices – suitable for food, beverage, botanical and nutraceutical products.
• Capability for technically challenging samples – including high-fat chocolates, concentrated botanical extracts, powdered supplements and fortified beverages.

Caffeine and theobromine are important indicators of product composition, quality, authenticity and regulatory compliance. Accurate and reliable quantification is essential to support label compliance, product consistency, consumer transparency and regulatory verification.

The method delivers high analytical sensitivity, excellent chromatographic separation and robust extraction performance, enabling reliable quantification across a wide range of matrices, including technically challenging sample types.

Next steps:
If your organisation would like to use this testing capability, our team is ready to support you, simply submit a request.